Seed Enhancements

We utilize a suite of techniques known as Seed Enhancement Technologies – SETs.

Key to SETs is the germination profile of the seed, which establishes the conditions and moisture needed to begin germination. The germination profile of every seed has three phases:

Phase 1

The seed absorbs water.

Phase 2

With sufficient water, and warmth, the internal metabolic processes that lead to germination begin.

Phase 3

The radicle emerges.

seedballs sprouting
seed acornball
seedballs volume stacked
seedballs volume

The germination profile we establish for each species becomes the blueprint for its priming protocol.

Priming works by starting seeds on their path toward germination, then pausing them just before sprouting – at the end of Phase II. In this state, the seeds are poised on the cusp of germination. They can be safely stored while awaiting favorable conditions. When rain and warmth arrive, they germinate rapidly and synchronously, taking immediate advantage of the window Nature provides.

The time they take to germinate matters because one of restoration’s greatest challenges is the length of that time. Seeds that do not germinate quickly remain on the soil surface. Exposed, they are vulnerable to drought, disease, and a host of predators. In most direct seeding efforts the seeds are lost before they ever have a chance to grow. With costly or hard-to-source seeds, this loss becomes a serious barrier to effective restoration.

As a result, practitioners often turn to planting plugs instead – a method that is labor-intensive, costly, and difficult to scale.

Our Seed Enhancements address this challenge directly.

  • Germinate faster and more uniformly

  • Germinate reliably in narrow weather windows

  • Compete more effectively with invasive species

SETs for Scalable Restoration

By improving early establishment, our enhancements help restoration practitioners achieve higher success rates with fewer inputs, making large-scale habitat recovery more feasible, economical, and effective.

Species-Specific Enhancements in Progress

We are currently developing and validating Solid Matrix Priming (SMP) protocols for the following eight native species, selected for their ecological importance, restoration demand, and scalability potential. Protocol readiness reflects the stage at which protocols are field-tested and ready for dissemination.

  • Asclepias fascicularis (narrowleaf milkweed) — Ready April 2026
  • Asclepias speciosa (showy milkweed) — Ready June 2026
  • Stipa pulchra (purple needlegrass) — Ready September 2026
  • Phacelia imbricata (imbricate phacelia / Pine Bee Flower) — Ready December 2026
  • Festuca rubra (red fescue) — Ready 2027
  • Helianthus californica (california sunflower) — Ready 2027
  • Symphyotrichum chilense (pacific aster) — Ready 2027
  • Asclepias cordifolia (heartleaf milkweed) — Ready 2028

Each protocol is tailored to the biological and physiological needs of the species, rather than relying on one-size-fits-all treatments.

hands holding small plant

Help Us Scale This Work

Developing protocols is only the first step. Dissemination is critical. To achieve real impact, these enhancements must reach the people restoring land on the ground—at scale.

Diversity of species is also critical. With the support of the California Wildlife Conservation Board, we have so far been able to fund the development of priming protocols for eight key species.

Please help us fund more.

We are seeking partners, sponsors, and donations to help:

  • Translate protocols into practitioner-ready formats
  • Share methods through trainings, pilots, and demonstrations
  • Integrate enhancements into regional and statewide restoration efforts
  • Expand access to tools that make restoration more effective and affordable

If you are a land manager, researcher, organization, or funder interested in collaborating—or helping carry these tools further—we invite you to connect with us.

Together, we can turn proven science into living landscapes.

All donations are secure and tax-deductible.
Rainforest Rising is a non-profit 501(c)3, EIN# 27-4723321

Our current focus is narrowleaf milkweed (Asclepius fascicularis), including observations to enable large-scale generation of monarch and pollinator habitat. We also profile other keystone species including native California grasses for rapid post-fire restoration (Stipa pulchra, Danthonia californica, and Festuca california), and longleaf pine (Pinus palustris).

Narrowleaf milkweed is a Sonoma-native host plant for monarch caterpillars and our primary research flower. In much of North America narrowleaf used to be common, but extensive eradication campaigns have made her rare. She is drought-tolerant but vulnerable while young. After establishment she is hardy. She produces seeds but is also rhizomatous, sprouting new growth from her roots. With favorable conditions she can be prolific. She is a perennial and lives 10-20 years, dying back every fall and resurfacing in mid-spring.

Our narrowleaf research indicates:
  • Phase 1 is complete at ~48% seed moisture content
  • Time to germination: 48-240 hours
  • Fungus vulnerability low (due to rapid germination)
  • Seeds per pound: 100,000 – 150,000
  • Preferred substrates: peat and sand. Other trials were done with clay, biochar, vermiculite, perlite, worm castings, compost, and combinations of these. The highest germination rates resulted from peat.
Phase 1:
research phase 1 fascicularis imbibition

Phase 1 of this lot of seeds is completed in ~5 hours: the moisture content has stabilized and the seed has stopped imbibing.

Phase 2:
research phase 2 fasc mcfw vs hours

Phase 2 stabilizes at ~48% moisture content: the seed has imbibed nearly half its weight in water and has now entered Phase 2.

Fascicularis germination trials:
research germination in peat pellets
research chart fasc sand substrate 01
research chart fasc sand substrate 02
Field Capacity (FC) trials test the amount of moisture in the substrate:
research chart fc trial 1
research chart fc trial 2
research chart fc trial 3
Cold stratification is a priming strategy that simulates winter to break seed dormancy. Here we test 10-day and 20-day stratification. FC = amount of substrate moisture.
research chart 10 day cold strat
research chart 20 day cold strat
We optimize seed moisture content in storage to keep seeds viable over time.
research chart storage weight seed
research chart palustris seed weight
research chart pulchra seed weight
We test pod formulae with varying ingredients, ratios, moisture contents, conditions, etc. These trials test germination rates in seedpods with varying amounts of nutrients. Here are the high-performing formulas we have trialed for narrowleaf milkweed.
research chart formula 1.2
research chart formula 2.3
research chart formula 3.3
Compilation of fascicularis trials:
research chart compilation fascicularis trials 1
research chart compilation fascicularis trials 2
research chart compilation fascicularis trials 3
Other species:
Longleaf Pine (Pinus palustris)
research chart palustris germination 50 seeds
Testing Predator Deterrent: Cayenne
research chart palustris w cayenne
Purple Needlegrass (Stipa pulchra):
research chart pulchra phase 1
research chart stipa pulchra sand
Testing cayenne as a predator deterrent
research chart palustris germination test priming predator deterrent
Showy milkweed (Asclepius speciosa)
research chart speciosa phase 1
Testing seedpod materials for moisture retention:
research chart moisture retention 1
research chart moisture retention 2
dark drone isolated
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